Figure 2.

(A) mSPP1 complements the hypersensitivity of Δlbp1 to australifungin. Δlbp1 mutant transformed with control vector (■), or mSPP1 in pRS414-ADH (○), or wild-type strain W3031a (●) were tested for their sensitivity to australifungin. Data are expressed as percent of cell growth in the absence of inhibitor. (B) Transformation of yeast with mSPP1 enhances long-chain base phosphate phosphohydrolase activity. Phosphohydrolase activity was measured in Δlbp1 mutants carrying vector, or mSPP1 on pRS414-ADH, or in wild-type cells as described in Materials and Methods. Results are means ± SD of quadruplicate determinations.