FIG. 2.

In vivo binding of NF-Y and Sp1/Sp3 to FAS promoter. (A) PCR analysis of immunoprecipitates of liver samples from fasted and refed −444 FAS-CAT transgenic animals when 5 μl of anti-acetylated-H3, 5 μl of anti-acetylated-H4, and 30 μg of anti-NF-Y antibodies were used. ChIPs shown here are representative of at least two independent experiments. (B) Same as panel A, but using 30 μg of anti-Sp1, 4 μg of anti-Sp3, and 30 μg of anti-NF-Y antibodies.