FIG. 4.

The −131 FAS promoter does not show binding of SREBP in vivo during refeeding. (A) Livers from fasted and refed −131 FAS-CAT transgenic mice were cross-linked, and the chromatin was isolated, sonicated, and immunoprecipitated with antibodies against the indicated proteins. The DNA was analyzed by PCR with primers for the specified gene promoters. Binding to the LDL receptor (LDLR) promoter is provided as a control. (B) Same as panel A, but the liver samples were obtained from fasted and refed −131 FAS-CAT/SREBP double-transgenic mice. The results shown here are representative of at least three independent experiments.