Skip to main content
. 2003 Aug;23(16):5896–5907. doi: 10.1128/MCB.23.16.5896-5907.2003

FIG. 5.

FIG. 5.

USF and SREBP binding to the FAS promoter during nutritional regulation. (A) Cross-linked chromatin of livers from fasted and refed −278 FAS-CAT transgenic mice was immunoprecipitated with anti-USF and anti-SREBP antibodies, and the DNA pulled down with the antibody-transcription factor complexes was analyzed for the indicated gene promoters by PCR. Binding of the indicated proteins to the LDL receptor (LDLR) promoter is shown as a control. (B) Livers from −278 FAS-CAT mice refed for the specified times were dissected, cross-linked, and immunoprecipitated with anti-USF and anti-SREBP antibodies, and the DNA was analyzed by PCR with specific primers for the transgene FAS promoter. (C) Same as panel A, but the ChIP was performed on livers from fasted and refed −278 FAS-CAT/SREBP double-transgenic animals. The results shown here are representative of at least two independent experiments.