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. 2003 Aug;23(16):5896–5907. doi: 10.1128/MCB.23.16.5896-5907.2003

FIG. 7.

FIG. 7.

The −65 E-box is necessary for FAS transcriptional regulation in vivo. (A) The diagram shows the mutation introduced at the −65 E-box within the context of the −444 bp FAS promoter used to generate the −444(−65m) FAS-CAT transgenic mice. (B) RPA was performed with mRNA extracted from the livers of fasted and refed −444(−65m) FAS-CAT transgenic animals to determine the mRNA levels for endogenous FAS and the reporter CAT. β-Actin mRNA levels are shown as controls. Three transgenic lines were used in this experiment. (C) Immunoprecipitation was performed on cross-linked chromatin of livers from fasted and refed −444(−65m) FAS-CAT transgenic mice with antibodies against the indicated proteins. DNA was analyzed by PCR with the appropriate primers. The LDL receptor (LDLR) promoter is shown as a control. The results shown are representative of a minimum of three independent experiments for at least two of the founder lines.