Fig. 2.

Dipeptide formation. (A) TLC analysis of reaction products. Lanes 1 and 2, reaction of [¹⁴C]Ala-minihelix with 5′-Phe-p-dT₆dA₂ in the absence (lane 1) or presence (lane 2) of template oligonucleotide (5′U₂A₆UGGU3′). Lane 3, a “mismatched” template oligonucleotide (5′U₂A₆CCCC3′) was used. Lane 4, reaction of [¹⁴C]Ala-minihelix with 5′-Phe-p-dT in the presence of template oligonucleotide (5′AUGGU3′). (B) MS of reaction products produced from Ala-minihelix, template oligonucleotides, and 5′-Phe-p-oligonucleotide (A, lane 2). The peak at 237 mass units corresponds to Phe-Ala.