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. 2003 Jun 30;100(15):8670–8675. doi: 10.1073/pnas.1432840100

Fig. 4.

Fig. 4.

In vitro pull-down assay of purified Kv3 and Kv4 T1 mutants. Below the gels, bait proteins Kv3(WT), Kv3(A63R), and Kv4(WTL) that are tagged by N-terminal 6-histidine tags are shown (Upper). Molecular weights of these bands range from ≈12 to ≈15 kDa. To test self-association of Kv4, a Kv4 WT with a 16-aa C-terminal extension [Kv4(WTL)] was used. The difference in size allowed us to distinguish them by SDS/PAGE. (Lower) The test proteins [Kv4(WT) and Kv4(R93A)] that are not histidine-tagged. Black bars represent the presence of those bait and test proteins in the reaction mixtures. The presence of the corresponding bands in the gels indicates the assembly affinity between them.