Fig. 3.
The behavior of the tubule conductance during the full cycle of the shape transformations; D1/D2 ≈ 50, D2 ≈ 1 μm. (A) The decrease in conductance measured during the WT elongation (as in I in Fig. 2 A). Initial conductance corresponds to the patch-pipette resistance (see Fig. 2 A). Circles indicate averaged experimental data, and the red line shows the calculated conductance of the catenoid. The fitting procedure allowed us to determine Lc with ≈5% precision. (B) The conductance decrease during the WT-to-NT transformation at fixed L ≈ Lc; conductance “ringing” oscillations are seen. (C) The NT conductance growth during the decrease of L (as in II in Fig. 2 A). Circles indicate averaged experimental data, and the red line shows the hyperbolic fit to the data. (D) Small conductance fluctuations preceding the NT-to-WT transformation and the final increase of the conductance (as in II in Fig. 2 A). (Inset) The behavior of the NT conductance during the decrease of L, OPC/OPE/Chol membrane tubule.