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. 2000 Jul 5;97(14):7877–7881. doi: 10.1073/pnas.97.14.7877

Figure 1.

Figure 1

Sequence analysis. (A) Schematic representation of transcripts from the sel-8 gene. RACE analysis revealed two potential transcripts, sel-8A and sel-8B. The sel-8(sa54) C-to-T mutation is indicated (nucleotide 17,295 of cosmid C32A3, GenBank accession no. Z48241). Introns are not drawn to scale. (B) Schematic representation of the SEL-8 protein. The “SEL-8 motif” that is found in W09C5.1 and its orthologs, the PEST sequence, and the two glutamine-rich regions are indicated and described further in the text. (C) Amino acid sequence of SEL-8. The sequence based on the sel-8A RACE product is shown. The PEST sequence is underlined with a solid line, and the motif shared with W09C5.1 is underlined with a dashed line. Glutamine (Q) residues are indicated in bold type. The boxed glutamine corresponds to codon 387 of sel-8A, which is changed to “stop” in sel-8(sa54). (D) Sequence motif found in SEL-8 and W09C5.1 (GenBank accession no. T26298) and its apparent yeast (YER126c, GenBank accession no. S43218) and Drosophila (DMIP259, GenBank accession no. AAF52940) orthologs. Reverse-contrast lettering indicates amino acids that are identical in SEL-8 and at least two of the other proteins.