Figure 3.

Proliferative effect of ET3 on pigment cells in clonal cultures. (A) Time course of the onset of cell division: the proportion of clonal cultures in which the founder pigment cell has divided was determined as a function of the duration of the culture period and compared between control and ET3-supplemented medium. (B) Histogram of the distribution of d13-pigment cell colonies according to their total number of cells. About 70% of control clones contained 1–10 cells only whereas 76% of ET3-treated colonies (versus 15% of control clones; P < 0,0001) contained more than 100 cells.