Table 2.
Phenotypic analysis of pigment cell colonies
| Control | + ET3 | P | |
|---|---|---|---|
| Total number of clones | 142 | 165 | |
| Total number of pigm+ clones | 141 (99.3%) | 159 (96.4%) | ns |
| Total number of SMP+ clones | 12 (8.4%) | 68 (41.2%) | 0,0001 |
| Total number of clones | 77 | 89 | |
| Number of Mel+ Po−SMP− clones | 72 (93.5%) | 40 (44.9%) | 0,001 |
| Number of Mel+ Po+SMP− clones | 1 (1.3%) | 23 (25.8%) | 0,005 |
| Number of Mel+ Po−SMP+ clones | 0 (0%) | 1 (1.1%) | ns |
| Number of Mel+ Po+SMP+ clones | 4 (5.2%) | 25 (28.1%) | 0,005 |
Cell phenotypes were analysed at d13 in control and ET3-treated colonies. The number of clones containing pigment cells (pigm+) and SMP+ cells was evaluated in four experiments and was expressed as percent of total clones. In two experiments, melanocytes (Mel), SMP+, and Po+ cells were recorded, and four different types of clones were defined according to the cell types they contain. P values are from statistical analysis of control and ET3-treated cultures. ns, not significant.