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Time course of thorax closure. (A) Dorsal view of a 5.5-h APF pucE69/+ pupa stained with anti-β-galactosidase and anti-spectrin (to visualize cell perimeters) antibodies. The most anterior cells of the wing imaginal disc are the first to meet at the midline. (B) The most posterior imaginal cells move forward and initiate fusion at 6 h APF. (C) Middle cells fuse at 6.5 h APF.
