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. 2003 Jul 11;100(15):8850–8855. doi: 10.1073/pnas.1033132100

Fig. 1.

Fig. 1.

Vector construction map, protein expression, bioactivity, and targeting of expression constructs. (A) The coding sequence of full-length, murine Fra-1, fused with polyubiquitin at the N terminus, was inserted into the pIRES plasmid (pUb-Fra-1). A second plasmid, pIL-18, contained the entire coding sequence of murine IL-18 with an Igκ leader sequence. Protein expression by pUb-Fra-1 and pIL-18 was demonstrated by Western blotting. Blots are shown at either pUb-Fra-1 (lane 1) or pIL-18 (lane 2) and of culture supernatant from pIL-18-transfected COS-7 cells (lane 3). (B) Bioactivity of IL-18 (ng/ml) determined by ELISA in supernatants of KG-1 lymphoma cells that transfected with pIL-18. The error bars indicate mean standard deviation of multiple assays. (C) Expression of EGFP activity in Peyer's patches was determined in mice immunized with 108 aroA– dam– bacteria transformed with pEGFP (S.T-GFP) by gavage. Fluorescence expression of EGFP was detected by confocol microscopy (Right). Hematoxylin/eosin staining of mouse Peyer's patches is shown (Left).