Fig. 2.
Effect of the pUb-Fra-1/pIL-18-based DNA vaccine on primary tumor growth and metastases. Each experimental group (n = 8) of BALB/c mice was vaccinated by oral gavage as described in Materials and Methods.(A) Suppression of experimental pulmonary metastases of D2F2 breast carcinoma. Results are expressed as metastatic scores, i.e., percentage of lung surface covered by fused tumor foci. (B) Tumor growth was analyzed in mice challenged s.c. with 1 × 106 D2F2 tumor cells 1 wk after the last vaccination in each treatment or control group. (C) Survival curves represent results for eight mice in each of the treatment and control groups. Surviving mice were tumor free unless otherwise stated.