Table 1. Summary of affinity constants of the interactions of ILT2D1D2 and ILT4D1D2 to MHCIs.
|
Injected, Kd at 25°C, μM
|
|||
|---|---|---|---|
| Immobilized | sILT2D1D2 | sILT4D1D2 | CD8αα |
| HLA-A11 | ND | 45 ± 17 (3) | ND |
| HLA-B35 | 8.8 ± 0.2 (5) | 26 ± 4.6 (8) | 126 ± 3 (4) |
| HLA-Cw4 | 6.5 ± 0.5 (5) | 14 ± 2.0 (4) | 210 ± 10 (2) |
| HLA-G1 | 2.0 ± 0.7 (11) | 4.8 ± 1.4 (10) | 72 ± 1.4 (4) |
| HLA-Cw7/DS11* | NB | 26 ± 6.0 (8) | ND |
| HLA-Cw7/DS12* | NB | 23 ± 6.2 (4) | ND |
Shown is the mean ± standard deviation. The number of measurements is shown in parentheses. The immobilized levels of MHCs are from 800 to 3,000 response units. NB, no binding observed at the ILT2D1D2 concentration of 87 μM; ND, not determined.
*
Chemically biotinylated β2m was used.