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(A) Folding of hBD1 and hBD2 aided by cysteine/cystine. Fully reduced, purified peptides were analyzed by C18 RP-HPLC (thin lines) with a linear gradient of 5–65% acetonitrile over 30 min. Peptides folded after 24 h (thick lines) were analyzed under the same chromatographic conditions. (B) Folding of hBD3 under various oxidative conditions: DMSO, air, and cysteine/cystine. Analyses by C18 RP-HPLC were carried out using a linear gradient of 20–35% acetonitrile over 30 min.
