Fig. 1.

(A) Transcription of LEE::lacZ fusions integrated into the K-12 chromosome in fresh medium (LB) and medium preconditioned by growth of WT strain 86-24, the luxS mutant (VS94), or the complemented mutant (VS95). (B) Induction of luminescence in V. harveyi strain BB170 by fecal filtrates from volunteers from the Center for Vaccine Development at the University of Maryland School of Medicine. As positive and negative controls we used PC medium with 86-24 and DH5α (which does not produce AI-2), respectively.