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. 2003 Jul 7;100(15):8951–8956. doi: 10.1073/pnas.1537100100

Fig. 2.

Fig. 2.

(A) Western blot of type III-secreted proteins from strains 86-24, VS94, and VS95 in fresh DMEM; secreted proteins from VS94 in DMEM preconditioned with HeLa cells, nonpreconditioned DMEM + 10% FBS, DMEM + 50 μM of Epi (E), or 50 μM of NE. (B) β-galactosidase activity of a LEE1::lacZ chromosomal fusion in K-12 grown in fresh DMEM to an OD600 ≤ 0.2 in the presence of 50 μM Epi, NE, PO, PE, gastrin (GA), galanin (GL), and secretin (S) or with no additives (M). (C) Western blot of secreted proteins from VS94 and 86-24 grown in fresh DMEM (M), DMEM + 50 μM Epi, DMEM + 500 μM PO, DMEM + 50 μM Epi and 500 μM PO (E/PO), DMEM + 500 μM PE, and DMEM + 50 μM Epi and 500 μM PE (E/PE). (D) Western blot of flagellin from 86-24, VS94, VS95, VS94 + PC medium with HeLa cells, and VS94 + 50 μM of Epi. (E) Motility in DMEM + 50 μM Epi of EHEC 86-24, luxS (VS94), and qseC (VS138) mutants. (F) Transcription of qseA::lacZ in fresh medium (M) and in DMEM + 50 μM of Epi, PE, and PO in WT and luxS– backgrounds. *, Transcription in the luxS mutant with PE and PO was performed in the presence of Epi; no Epi was added to the WT strain.