Fig. 1.

Expression of ICP0 and accumulation of PML in infected cells. (A) Accumulation of PML and ICP0 in three cells lines exposed to ratios of PFU per cell shown, incubated for 24 h after virus exposure, and processed as described in Materials and Methods. The 6% denaturing polyacrylamide gels were loaded with 40 μg of total protein per lane. The HSV-1(F) stock was titered in Vero cells. Mock, mock-infected cells; cells, cells maintained in growth medium and neither mock-infected nor infected. The electrophoretically separated proteins were reacted with antibodies as described in Materials and Methods. (B) PML degradation in SK-N-SH cells. Zero time is 1 h after initial exposure of cells to virus. Cells were harvested at 2 and 12 h after mock infection and at times shown after HSV-1(F) infection (0.5 PFU per cell). The harvested cells were processed as described in Materials and Methods and in the legend to A.