Fig. 2.

Accumulation of PML in SK-N-SH cells mock-infected or infected with HSV-1(F) 24 h after they were mock-transduced with insect cell medium or transduced with baculoviruses expressing wt or dn UbcH5a, UbcH6, or UbcH7. The multiplicities of infection were 10 PFU of baculoviruses and 5 PFU of HSV-1(F) per cell. To enhance the expression of genes encoded in baculovirus vectors, the cultures were incubated in medium containing 5 mM sodium butyrate after transduction. The cells were harvested 30 min after the 1-h exposure to HSV-1(F), electrophoretically separated in a denaturing 4–20% gradient polyacrylamide gel, and reacted with antibodies as described in Materials and Methods. Actin served as a gel loading control.