Fig. 3.
Defects in development of telencephalic cholinergic neurons in P0 Lhx8 mutants (B, D, F, H, and J) as compared with wild-type controls (A, C, E, G, and I). (A–F) Anti-ChAT staining of the caudate-putamen (A and B), nucleus basalis (C and D), and magnocellular preoptic nucleus (E and F). (G and H) Anti-TrkA staining of the magnocellular preoptic nucleus. Note that the number of labeled cells is reduced in Lhx8 mutants. (I and J) In situ analysis showing that the expression of Gbx1 mRNA was maintained in the nucleus basalis and magnocellular preoptic nucleus in Lhx8 mutants. CP, caudate-putamen; GP, globus pallidus; MPN, magnocellular preoptic nucleus; NB, nucleus basalis. (Scale bar in J represents 180 μm for all panels.)