TABLE 1.

Sources of two-component mutants

Mutationa	Strain	Parent or controlb	Methodc
ΔarcA43	BW27422	BW25113	a
ΔarcA45	BW29409	BW28357	a
ΔarcB41	BW26422	BW25113	a
ΔarcB41	BW29859	BW28357	b
ΔatoSC571	BW29009	BW25113	a
ΔatoSC573	BW29010	BW25113	a
ΔbaeSR579	BW27553	BW25113	b
ΔbaeSR610	BW29744	BW28357	b
ΔbarA614	BW27563	BW25113	b
ΔbarA1358	BW29434	BW25113	a
ΔbasSR616	BW27549	BW25113	b
ΔbasSR1287	BW27848	BW25113	a
ΔbasSR620	BW29371	BW28357	a
ΔcheZYBA1218d	BW28079	BW25113	a
ΔcheZYBA1218	BW29661	BW28357	c
ΔcpxAR623	BW27559	BW25113	b
ΔcpxAR623	BW29849	BW28357	c
ΔcreABCD154	BW26983	BW25113e	d
ΔcreABCD176	BW29135	BW25113	a
ΔcusSR1204	BW30048	BW28357	c
ΔcusSR1204	BW30049	BW28357	c
ΔdcuRS(yjdGH)1330	BW27878	BW25113	a
ΔdcuRS(yjdGH)1330	BW29657	BW28357	c
ΔdpiBA(citAB)1289	BW27876	BW25113	a
ΔdpiBA(citAB)1289	BW29656	BW28357	c
ΔevgAS1291	BW27869	BW25113	a
ΔevgAS1291	BW29663	BW28357	c
ΔfimZ1214	BW28078	BW25113	a
ΔfimZ1214	BW29659	BW28357	c
ΔkdpEDCBAF1224	BW27564	BW25113	b
ΔkdpEDCBAF1224	BW30166	BW28357	c
ΔnarLX1316	BW27864	BW25113	a
ΔnarLX1316	BW29658	BW28357	c
ΔnarP1312	BW27873	BW25113	a
ΔnarP1312	BW30265	BW28357	c
ΔnarQ1314	BW27865	BW25113	a
ΔnarQ1314	BW30008	BW28357	c
ΔntrCB(glnGL)1318	BW27880	BW25113	a
ΔntrCB(glnGL)1318	BW30011	BW28357	c
Δ(envZ ompR)520	BW26424	BW25113	a
Δ(envZ ompR)520	BW29655	BW28357	c
ΔphoBR580	BW24476	BW25113e	d
ΔphoBR758	BW29134	BW25113	a
ΔphoBR758	BW30046	BW28357	c
ΔphoQP1244	BW27558	BW25113	b
ΔphoQP1244	BW30007	BW28357	c
ΔqseBC(ygiXY)1302	BW27551	BW25113	b
ΔqseBC(ygiXY)1304	BW29747	BW28357	b
ΔrcsB1320	BW27870	BW25113	a
ΔrcsB1320	BW30009	BW28357	c
ΔrssB1275	BW29745	BW28357	b
ΔrssB1273	BW30418	BW25113	c
ΔrssB1273	BW30419	BW25113	c
ΔrssB1273	BW30420	BW30383	c
ΔrssB1273	BW30421	BW30383	c
ΔrstAB1278	BW27552	BW25113	b
ΔrstAB1280	BW29806	BW28357	b
ΔtorSTRCAD518	BW26423	BW25113	a
ΔtorSTRCAD518	BW29855	BW28357	c
ΔuhpBA1322	BW27871	BW25113	a
ΔuhpBA1322	BW29852	BW28357	c
ΔuvrY1310	BW29475	BW25113	a
ΔuvrY1362	BW29476	BW25113	a
ΔyedVW1298	BW27550	BW25113	b
ΔyedVW1300	BW29746	BW28357	b
ΔyehTU1324	BW27877	BW25113	a
ΔyehTU1324	BW29858	BW28357	c
ΔyfhA1326	BW27868	BW25113	a
ΔyfhA1326	BW29851	BW28357	c
ΔyfhK1328	BW27872	BW25113	a
ΔyfhK1328	BW29857	BW28357	c
ΔyojN1332	BW27866	BW25113	a
ΔyojN1332	BW29856	BW28357	c
Δ(yojN rcsBC)1308	BW27557	BW25113	b
Δ(yojN rcsBC)1308	BW29861	BW28357	c
ΔypdAB1334	BW27875	BW25113	a
ΔypdAB1334	BW29860	BW28357	c
ΔzraSR(hydHG)1336	BW27867	BW25113	a
ΔzraSR(hydHG)1336	BW29660	BW28357	c

^aCommon alternative nomenclature is indicated in parentheses. Different alleles are used to identify mutations that differ with regard to the template or primer used for generation. The allele assignments for multiple gene deletions are in clockwise order in accordance with the K-12 map. Mutants are available from the Coli Genetic Stock Center (http://cgsc.biology.yale.edu/). Genes are designated according to their order within an operon.

^bE. coli K-12 strains BW28357 and BW30383 are independent rph⁺ derivatives of BW25113 that were made by using the Red system and P1 transduction, respectively (K. A. Datsenko and B. L. Wanner, unpublished data).

^cMutants were made in one of four ways. In method a, mutants were isolated as kanamycin-resistant derivatives of the parent carrying pKD46 following introduction of a PCR fragment generated with long (56- to 60-nt) primers and pKD13 as the template, after which the resistance marker was eliminated with pCP20 as described elsewhere (10). In method b, mutants were isolated as kanamycin-resistant transductants by using P1kc grown on the corresponding kanamycin-resistant mutant, after which the resistance marker was eliminated with pCP20. In method c, mutants were isolated as kanamycin-resistant derivatives of the parent carrying pKD46 following introduction of a PCR fragment generated with locus-specific test primers and the corresponding kanamycin-resistant mutant as the template, after which the resistance marker was eliminated with pCP20. In method d, the mutation was constructed on a conditionally replicative plasmid by using standard cloning techniques and then recombined onto the chromosome by using our standard two-step allele replacement method (45), after which the mutation was transferred to a parent by cotransduction with nearby proC⁺ and thr⁺ markers for the phoBR and creABCD loci, respectively.

^dThe ΔcheZYBA1218 mutation has a deletion of several nearby genes and corresponds to the Δ(flhEAB cheZYBR tap tar cheWA motBA flhCD IS1)1218 mutation.

^eBW24476 and BW26983 are descendents of strains like BW25113 and served as controls.