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Cobalamin-dependent growth of metE strains with various levels of TonB expression
| Strain/plasmid | Relative TonB level | Growth (mean diam [mm] ± SD) with:
|
||
|---|---|---|---|---|
| 27 mM methionine | 100 nM cobalamin | 500 nM cobalamin | ||
| KP1402/pACYC184 | 0.0 | 27 ± 0.5 | No growth | No growth |
| KP1033/pACYC184 | 1.0 | 26 ± 0.5 | 11 ± 0.5 | 19 ± 0.5 |
| KP1413/pACYC184 | 1.0 | 27 ± 0.5 | 11 ± 0.5 | 20 ± 0.8 |
| KP1402/pKP325 | 0.13 | 26 ± 0.5 | 13 ± 0.5 | 24 ± 0.5 |
| KP1407/pKP325 | 0.004 | 29 ± 1.0 | 20 ± 0.5 | 27 ± 0.0 |
| KP1402/pKP299 | 8.0 | 27 ± 0.5 | 14 ± 0.5 | 21 ± 0.5 |
