TABLE 7.
Sensitivity to bacteriophage φ80 of aroB strains with various levels of TonB expression
| Strain/plasmid | Relative TonB level | Sensitivity
|
|
|---|---|---|---|
| Spot titera | Relative titer (PFU) | ||
| KP1406/pACYC184 | 0.0 | R R R | 0 |
| KP1270/pACYC184 | 1.0 | 888 | 2.8 × 108 ± 0.1 × 108 |
| KP1414/pACYC184 | 1.0 | 778 | 2.2 × 108 ± 0.1 × 108 |
| KP1406/pKP325 | 0.11 | 887 | 2.1 × 108 ± 0.1 × 108 |
| KP1408/pKP325 | 0.005 | 776 | 4.8 × 106 ± 0.4 × 106 |
| KP1406/pKP299 | 9.0 | 877 | 2.7 × 108 ± 0.1 × 108 |
a
Scored as the highest 10-fold dilution of a phage preparation that provided an evident zone of clearing on a cell lawn. R indicates resistance (i.e., no clearing) with undiluted phage. For spot titers, the values of three platings are presented for each strain/plasmid and phage pairing.