TABLE 3.
Min protein interactions in yeast two-hybrid system
| Fusion to BDa | Fusion to ADa,b | Color on X-Gal platec | β-Galactosidase activityd |
|---|---|---|---|
| −b | MinD | 0 | <0.2 |
| MinE1-88 | − | 0 | ND |
| MinE1-88 | MinD | ± | 0.9 ± 0.09 |
| MinE1-31 | − | 0 | ND |
| MinE1-31 | MinD | +++ | 112 ± 5 |
| MinE32-88 | − | 0 | ND |
| MinE32-88 | MinD | 0 | <0.2 |
| MinE1-31 | MinE32-88 | 0 | <0.2 |
| MinE1-31 | MinE1-31 | 0 | <0.2 |
| MinE32-88 | MinE32-88 | 0 | <0.2 |
| MinD | MinD | ++ | 42 ± 1 |
| MinC | MinD | ++++ | 715 ± 5 |
| MinC | MinC | + | 6 ± 1 |
a
See Materials and Methods and Table 1 for names of plasmids.
b
−, no gene fusion.
c
0, white colonies; ± to ++++, intensity of blue color of colonies.
d
Miller units. ND, not done.