Table 2.

Synthetic Interactions between Δabp1 and DNA replication mutants.

S. pombe strains	Gene product	25°C	30°C	31°C	32°C	33°C	34°C	36°C
Wild type		++	++	++	++	++	++	++
Δabp1		+	+	+	+	+	+	+
cdc23-M36	S phase factor	++	++	++	-	-	-	-
Δabp1 cdc23-M36		+	-	-	-	-	-	-
Δabp1Δcbh2		+	+	+	+	+	+	+
Δabp1Δcbh2 cdc23-M36		+	-	-	-	-	-	-
Δcbh1Δcbh2		+	+	+	+	+	+	+
Δcbh1Δcbh2 cdc23-M36		+	+	+/-	-	-	-	-
cdc20-P7	DNA polymerase	++	++	++	++	++	-	-
Δabp1 cdc20-P7	ε catalytic subunit	+	+	+	+	+	-	-
cdc20-M10	DNA polymerase	++	++	++	++	++	+/-	-
Δabp1 cdc20-M10	ε catalytic subunit	+	+	+	+	+	+	-
cut5-T401	S phase initiator	++	++	++	++	++	-	-
Δabp1 cut5-T401		+	+	+	+	+	-	-
cdc30-2H4	ORC complex	++	++	++	+/-	-	-	-
Δabp1 cdc30-2H4		+	+	+/-	-	-	-	-
cdc18-K46	S phase initiator	++	++	++	++	++	+	-
Δabp1 cdc18-K46		+	+	+	+	+/-	-	-
cdc21-M68	MCM2-7 complex	++	++	++	+	+	-	-
Δabp1 cdc21-M68	subunit 4	+	+	+	+/-	-	-	-
sna41-912	S phase initiator	++	++	+/-	-	-	-	-
Δabp1 sna41-912		+	+	+/-	-	-	-	-
orp2-2	ORC complex	++	++	++	++	+	-	-
Δabp1 orp2-2		+	+	+	+	+	+	-
orp2-7	ORC complex	++	++	++	++	++	++	+
Δabp1 orp2-7		+	+	+	+	+	+	-
cdc1-P13	DNA polymerase	++	++	-	-	-	-	-
Δabp1 cdc1-P13	δ small subunit	+	+	+	-	-	-	-
cdc6-23	DNA polymerase	++	-	-	-	-	-	-
Δabp1 cdc6-23	δ catalytic subunit	+	+/-	-	-	-	-	-
cdc27-P11	DNA polymerase	++	++	++	++	-	-	-
Δabp1 cdc27-P11	δ associated factor	+	+	+	+	+/-	-	-
cdc17-M75	DNA ligase	++	++	+	+/-	-	-	-
Δabp1 cdc17-M75		+	+	+	+	+	+/-	-

Note: ++ = normal growth; + = slow growth; +/- = very slow growth, elongated; = dead, elongated