TABLE 2.
Growth of the P. putida tol-oprL mutants with different carbon sources
| Carbon source | Growth of P. putida strainsa
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| KT2440 | QΩ | RΩ | AΩ | BΩ | QX | RX | AX | BX | DOT-OX2 | |
| Arginine | ++ | − | − | − | − | − | − | − | − | − |
| Benzoate | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
| Fructose | +++ | − | − | − | − | − | − | − | − | − |
| Glucose | +++ | + | + | + | + | + | ++ | ++ | + | ++ |
| Glycerol | +++ | − | − | − | − | − | − | − | − | − |
| Proline | +++ | − | − | + | + | ++ | ++ | ++ | + | ++ |
| Succinate | ++ | + | − | + | ++ | + | + | + | ++ | + |
| Sucrose | ++ | + | − | + | + | − | − | + | + | + |
Culture cell density was determined after 23 h of incubation at 30°C in M9 minimal medium supplemented with the different carbon sources. +++, OD660 ≥ 2; ++, 0.7 ≤ OD660 < 2; +, 0.2 ≤ OD660 < 0.7; −, OD660 < 0.2. The initial OD660 of the cultures were 0.06 to 0.1.