Table 2.
Summary of screening results of the giant panda BAC library by PCR with primers for functional genes
| Gene | Genebank accession No. | Primers (5'-3') | Ta (°C) | PCR product size (bp) | Positive clone number |
| ACTIVIN | AF350256 | F- GGACATTGGCTGGAATGACT R- GACATGGGTCTCAGCTTGGT |
54 | 207 | 4 |
| AGXT | AJ539311 | F- CTGTTTTTCATTGACTGGACTCTG R- TCTCATCCATTATCTGGGGG |
58 | 539 | 4 |
| GHR | AF367203 | F- CCTCTGGGGGAAAAAAAGTT R- TCATCATCCTTTGCTCCAAG |
56 | 412 | 8 |
| FSH | AF448454 | F- TGTTGCTGGAGAGCAATCTG R- TACGCCAGCTCTTTGAAGGT |
54 | 197 | 7 |
| FSHR | AF415208 | F- GCCAAAGGATTTGACACGAT R- TGGTGACCAGGATCATCAGA |
56 | 202 | 6 |
| GpH | AF448453 | F- GCCCAGAATGCAAGCTAAAG R- ACTGTGGCCTTGGTAAATGC |
54 | 190 | 5 |
| SOX14 | AF016377 | F- AACCCCAAGATGCACAACTC R- ACGTAGCCTCTTGGCCTCAC |
54 | 108 | 6 |
| IRBP | AY303836 | F- CCAGGAAGTGGTGAGCAAGC R- CGGTGAGGAAGAAGTCGGAC |
66 | 419 | 4 |
| TTR | AF039738 | F- TCTGCAGAGCTCACTGAACC R- TTCGATTAGCTGAGGGGAAA |
54 | 205 | 4 |
| BDNF | U56638 | F- TCTGGAGAGCGTGAATGGG R- CGATACAGGGACTTTTTCAAGG |
62 | 394 | 3 |
| GDNF | AF516767 | F- TGAAAAGGTCACCCGAGAAG R- AGCCGCTGCAGTACCTAAAA |
54 | 219 | 5 |
| NT-4 | AY189135 | F- AGCGAGACAGCACCAGCAA R- TCAGACACCCAGTGCCTCC |
64 | 263 | 5 |
| LH | AF448455 | F- GCTGTGGCTGCTGCTGAAC R- CCACAGGGAAGGAGACCATG |
58 | 313 | 2 |
| ZFX1 | AF016379 | F- TCGGTTCACACGAAGGACTAC R- TGATACACCTTCCTGCCACTG |
59 | 284 | 4 |
| ZFX2 | AF016380 | F- GGTGCAGCAACATGCTCTTA R- CCACGTGTTTCTTGAGTTCG |
54 | 188 | 4 |