Table 3.

Specific enzyme activities in E. coli strains BL21, BL21/pOri1 and BL21/pOri2 cultured in the chemostat system at different growth rates.

Enzymea	Activity (μmol min-1 mg-1)b,c
	BL21	BL21/pOri1	BL21/pOri2
HEK	0.030	0.100	0.110
GPI	2.290	4.660	4.630
PFK	0.240	0.640	0.600
FBP	0.014	0.043	0.049
FBA	1.210	2.960	2.810
GPD	0.026	0.051	0.052
TPI	1.700	4.400	4.410
PPC	0.220	0.430	0.440
PK	0.044	0.098	0.102
GPDH	0.350	0.120	0.070
PGL	0.280	0.210	0.120
PGD	0.150	0.090	0.080
RPE	0.320	0.150	0.100
RPI	0.540	0.230	0.030
TK	0.080	0.010	0.005
TA	0.120	0.070	0.020
CS	0.021	0.086	0.082
ICD	0.150	0.840	0.830
ICL	0.013	0.046	0.045
AH	0.090	0.190	0.210
OGD	0.022	0.088	0.810
SD	0.020	0.090	0.110
FH	0.021	0.097	0.110
MD	0.046	0.094	0.101
MAC	0.002	0.004	0.003
PCK	0.002	0.003	0.003

^a Abbreviations: HEK, Hexokinase; GPI, Glucose-6-phosphate isomerase; PFK, 6-phosphofructosekinase; FBP, Fructose-1,6-bisphosphatase; FBA, Fructose-bisphosphate aldolase; GPD, Glyceraldehyde-3-phosphate dehydrogenase; TPI, Triose phosphate isomerase; PPC, Phosphoenolpyruvate carboxylase; PK, Pyruvate kinase; GPDH, Glucose-6-phosphate dehydrogenase; PGL, 6-Phosphogluconolactonase; PGD, Phosphogluconate dehydrogenase; RPE, Ribulose-phosphate 3-epimerase; RPI, Ribose-5-phosphate isomerase; TK, Transketolase; TA, Transaldolase; CS, Citrate synthase; ICD, NADP⁺-specific isocitrate dehydrogenase; ICL, Isocitrate lyase; AH, Aconitate hydratase; OGD, Oxoglutarate dehydrogenase; SD, Succinic semialdehyde dehydrogenase; FH, Fumarate hydratase; MD, Malate dehydrogenase; MAC, Malic enzyme; PCK, Phosphoenolpyruvate carboxykinase.

^b The following growth rates were determined when bacteria were cultured in the batch cultivation: BL21, 0.46 h^-1; BL21/pOri1, 0.39 h^-1; BL21/pOri2, 0.29 h^-1.

^c The experiments were performed in triplicate and the results are shown as the average of three measurements with the standard deviation less than 5%.