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. 2006 Nov 17;5:34. doi: 10.1186/1475-2859-5-34

Table 4.

Specific enzyme activities in BL21, BL21/pOri1 and BL21/pOri2 strains grown in the chemostat system at the same growth rate.

Enzymea Activity (μmol min-1 mg-1)b
BL21 BL21/pOri1 BL21/pOri2
HEK 0.012 0.051 0.074
GPI 0.947 2.228 2.085
PFK 0.104 0.241 0.311
FBP 0.004 0.020 0.034
FBA 0.402 1.439 1.937
GPD 0.011 0.023 0.034
TPI 0.719 1.991 2.072
PPC 0.093 0.129 0.253
PK 0.017 0.047 0.043
GPDH 0.108 0.061 0.036
PGL 0.103 0.090 0.058
PGD 0.038 0.040 0.043
RPE 0.107 0.073 0.059
RPI 0.180 0.096 0.013
TK 0.033 0.003 0.003
TA 0.037 0.035 0.014
CS 0.007 0.040 0.055
ICD 0.064 0.361 0.404
ICL 0.005 0.022 0.021
AH 0.030 0.085 0.097
OGD 0.006 0.031 0.369
SD 0.006 0.044 0.067
FH 0.008 0.035 0.069
MD 0.018 0.043 0.065
MAC 0.001 0.001 0.002
PCK 0.001 0.001 0.002

a Abbreviations are as described in the footnote to Table 3.

b The growth rate of each culture was 0.2 h-1. The experiments were performed in triplicate and the results are shown as the average of three measurements with the standard deviation less than 5%.