Table 4.
Specific enzyme activities in BL21, BL21/pOri1 and BL21/pOri2 strains grown in the chemostat system at the same growth rate.
| Enzymea | Activity (μmol min-1 mg-1)b | ||
| BL21 | BL21/pOri1 | BL21/pOri2 | |
| HEK | 0.012 | 0.051 | 0.074 |
| GPI | 0.947 | 2.228 | 2.085 |
| PFK | 0.104 | 0.241 | 0.311 |
| FBP | 0.004 | 0.020 | 0.034 |
| FBA | 0.402 | 1.439 | 1.937 |
| GPD | 0.011 | 0.023 | 0.034 |
| TPI | 0.719 | 1.991 | 2.072 |
| PPC | 0.093 | 0.129 | 0.253 |
| PK | 0.017 | 0.047 | 0.043 |
| GPDH | 0.108 | 0.061 | 0.036 |
| PGL | 0.103 | 0.090 | 0.058 |
| PGD | 0.038 | 0.040 | 0.043 |
| RPE | 0.107 | 0.073 | 0.059 |
| RPI | 0.180 | 0.096 | 0.013 |
| TK | 0.033 | 0.003 | 0.003 |
| TA | 0.037 | 0.035 | 0.014 |
| CS | 0.007 | 0.040 | 0.055 |
| ICD | 0.064 | 0.361 | 0.404 |
| ICL | 0.005 | 0.022 | 0.021 |
| AH | 0.030 | 0.085 | 0.097 |
| OGD | 0.006 | 0.031 | 0.369 |
| SD | 0.006 | 0.044 | 0.067 |
| FH | 0.008 | 0.035 | 0.069 |
| MD | 0.018 | 0.043 | 0.065 |
| MAC | 0.001 | 0.001 | 0.002 |
| PCK | 0.001 | 0.001 | 0.002 |
a Abbreviations are as described in the footnote to Table 3.
b The growth rate of each culture was 0.2 h-1. The experiments were performed in triplicate and the results are shown as the average of three measurements with the standard deviation less than 5%.