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. 2000 Jun 20;97(14):7921–7926. doi: 10.1073/pnas.130194897

Figure 2.

Figure 2

Hybridization patterns of relevant probes to drug-sensitive HeLa cell lines. (A) The chromosome 5 paint (red) labels two normal 5s and the der(3p5q) translocation derivative, each of which carries a DHFR gene (detected with a DHFR-specific cosmid in green), as well as two i(5p) chromosomes. (B) The chromosome 3 paint detects a normal 3, the der(3p5q) translocation (which is DHFR-positive), two other translocation products, and two acrocentric derivatives. The two normal 5 homologues also are illuminated by the DHFR-specific cosmid. (C–E) FISH patterns of a mixture of the DHFR cosmid (green) and one of three other 5q marker cosmids (C5–92, C5–8, and C5–9, respectively; red). (F) A mixture of all four cosmids, with DHFR in green. (G) Consensus chromosome arrangements deduced as described in ref. 28.