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. 2003 Aug;185(16):4851–4860. doi: 10.1128/JB.185.16.4851-4860.2003

FIG. 2.

FIG. 2.

Primer extension mapping of the S. gordonii luxS transcription start site. Labeled cDNA from reverse transcription reactions was run next to luxS sequencing reaction products (lanes G, A, T, and C) generated with the same primer. Reverse transcription products from reaction mixtures containing total RNA isolated from wild-type S. gordonii (lane 1), the complemented luxS mutant (lane 2), E. coli DH5α(pSF151-luxS) (lane 3), and E. coli DH5α without a plasmid (lane 4) are shown. An expanded view of the complementary nucleotide sequence surrounding the transcription start site (+1) is shown, and the putative extended −10 hexamer is highlighted with a vertical bar.