Figure 3.

Microrheology of Litoria caerulea toe pad mucus using laser tweezers. (a) Bead displacement elicited by sinusoidal fluid movement at three different frequencies. (b) Sinusoidal fit of bead and fluid (=substrate) movement. Here, bead displacement was phase-shifted by 87.0° against the movement of the fluid. (c) Relationship of bead displacement amplitude and velocity (frequency) measured for toe pad mucus and pure water. The slopes indicate that this sample of toe pad mucus was 1.65 times more viscous than water.