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. 2003 Aug;185(16):4844–4850. doi: 10.1128/JB.185.16.4844-4850.2003

FIG. 3.

FIG. 3.

Estimation of the molecular mass of B. subtilis NAD kinase. Gel filtration was performed as described in Materials and Methods. The arrow indicates the elution volume (Ve) of pure B. subtilis NAD kinase. The following protein standards (Sigma) were used: thyroglobulin (669 kDa) (A), apoferritin (443 kDa) (B), β-amylase (200 kDa) (C), alcohol dehydrogenase (150 kDa) (D), albumin (66 kDa) (E), and carbonic anhydrase (29 kDa) (F).