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. 2003 Aug;185(16):4844–4850. doi: 10.1128/JB.185.16.4844-4850.2003

FIG. 4.

FIG. 4.

Effects of pH and temperature on the activity of B. subtilis NAD kinase. (A) Effect of pH on NAD kinase activity assayed as described in Materials and Methods with 100 mM sodium acetate (▴), Tris-HCl (•), and glycine/NaOH (▪). (B) Effect of temperature (T) on NAD kinase activity assayed as described in Materials and Methods. (C) Thermal stability of NAD kinase. The purified enzyme was incubated for 15 min at different temperatures, and the residual activity was assayed as described in Materials and Methods.