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Kinetic behavior of B. subtilis NAD kinase. Enzyme activity was assayed at 25°C and pH 7.8 as described in Materials and Methods. (A) ATP titration curve in the absence of QA (•) and in the presence of 100 μM QA (▪). (B) NAD titration curve in the absence of QA (•) and in the presence of 100 μM QA (▪). (C) poly(P) titration curve in the absence of QA (•) and in the presence of 200 μM QA (▪).
