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Effects of metal ions on activity of B. subtilis NAD kinasea
| Metal | Relative activity (%) |
|---|---|
| None | ND |
| MgCl2 | 100 |
| MnCl2 | 180 |
| CaCl2 | 104 |
| ZnCl2 | 89 |
| MgCl2 | 68 |
| CoCl2 | 15 |
| Cu(CH3COO)2 | 8 |
| Ni(NO3)2 | ND |
| FeSO4 | ND |
The effects of metal ions were investigated by assaying the activity in the reaction mixture described in Materials and Methods, in which 5 mM MgCl2 was replaced by a metal ion at a concentration of 1.0 mM. ND, not detected.
