FIG. 3.

Identification of Rgl11Y as a component of the C. cellulolyticum cellulosome. The cellulosomal components purified from a cellulose-grown were separated by SDS-PAGE (10% acrylamide), transferred to a nitrocellulose membrane, and incubated with antibodies raised against Rgl11Y. Lane 1, SDS-PAGE (10% acrylamide) of the C. cellulolyticum cellulosome proteins (the proteins corresponding to the three major polypeptides of the cellulosomes [scaffolding protein CipC and cellulases Cel9E and Cel48F] and Rgl11Y are indicated by arrows on the left); lane 2, molecular mass markers; lane 3, Western blot analysis of the C. cellulolyticum cellulosomes with antibodies raised against Rgl11Y.