Figure 1.

Effects of various coactivators on the activation of PPARα by Wy-14,643 and CLA. COS-1 cells were transiently transfected with pM/PPARα, GAL4 reporter, and coactivator expression plasmids or empty vector control (total DNA per well was held constant). Cells were treated for 6 hours with 50 μM Wy-14,643, 100 μM CLA, or DMSO. Luciferase activity was measured and corrected for transfection efficiency and extraction yield (n = 3). Relative values are corrected to untreated pcDNA3 bar (100%). Bars with different letters above are significantly different from each other (Tukey's multicomparison test, P < .05), representative of 2 independent experiments.