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. 2006 Jun 27;2006:69612. doi: 10.1155/PPAR/2006/69612

Figure 4.

Figure 4

Interaction between LXXLL peptides and PPARα in mammalian two-hybrid assay. COS-1 cells were grown in serum-free media for 12 hours before transfection with GAL4DBD(pM)/peptide fusions, VP16/PPARα, pFR-luciferase, and pRL-TK. Panel (a): cells were treated with 100 μM α-linolenic acid (ALA), linoleic acid (LA), docosahexaenoic acid (DHA), conjugated linoleic acid (CLA), octanoic acid (OCT), oleic acid (OA), 25 μM Wy-14,643 (Wy), 25 μM ciprofibrate (Cipro), or DMSO (0.1% v/v) for 6 hours. Panel (b): cells were transfected and treated as described above with DMSO, CLA, Wy, and also with 100 μM eicosatetraynoic acid (ETYA), bezafibrate (Beza), or clofibrate (Clo). Luciferase activity was measured and corrected for extraction yield and transfection efficiency (n = 3). Data was expressed relative to that of the pM-empty construct and the presented heat map was generated in GeneSpring (Agilent, Palo Alto, CA). Darker shading represents higher luciferase activity.