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Molecular analysis of pGIIIb and pGΔΔ transcripts. Total RNA from heart, cerebellum, eye, and skin of GIIIb/Rint or GΔΔ/Rint mice was analyzed by RT-PCR for the presence of GFP transcripts and for the inclusion of exon IIIb. −RT lanes were not subjected to reverse transcription. PCR products derived from exon IIIb inclusion and skipping transcripts are indicated by E-IIIb-GFP and E-GFP, respectively.
