TABLE 3.

Effects of rpsD14 and rpsL1408 alleles on growth parameters and streptomycin sensitivity

Strain	Relevant genotype	Generation timea (min)		Growth at 44°Cb		STR sensitivity (MIC)c (μg/ml)
		−STR	+STR	−STR	+STR
BS1482	Wild type	27		+	−	6
BS1483	rpsD14	49		−	−	1
BS1484	rpsL1408	42	44	+	−	128
BS1485	rpsD14 rpsL1408	35	41	+	−	64

^aGeneration times were determined from viable-cell counts of two culture aliquots taken at the beginning and the end of the logarithmic phase of growth in Luria-Bertani medium at 37°C with (+) or without (−) streptomycin (STR). The following standard equation was used: G = t/[3.3 log(b/B)], where G is the generation time, t is the time interval in minutes, B is the cell count at the beginning of the time interval, and b is the cell count at the end of the time interval used.

^bCells (from fresh overnight colonies grown on Luria-Bertani agar at 37°C) were streaked on Luria-Bertani agar and incubated overnight at 44°C. +, growth; −, no growth.

^cMICs of streptomycin (STR) were determined by using the Etest (AB Biodisk, Solna, Sweden) in accordance with the procedures described by the manufacturer.