Left column, pCa = 5.63; right column, pCa = 4.50. Upper panels, length change per hs; lower panels, force response. Fibre length, 3.15 mm; segment length, 1.05 mm; average sarcomere length in the segment, 2.40 μm; CSA, 5800 μm2; temperature, 12.1°C; isometric force at pCa = 4.50, 66 kPa.