Table 1.
Composition of solutions used for human skinned muscle fibres
| Solution | TES (mM) | Na2ATP (mM) | MgCl2 (mM) | EGTA (mM) | HDTA (mM) | CaEGTA (mM) | Na2CP (mM) | GLH (mM) |
|---|---|---|---|---|---|---|---|---|
| Relaxing | 100 | 5.4 | 7.7 | 25 | – | – | 19.1 | 10 |
| Pre-activating | 100 | 5.5 | 6.9 | 0.1 | 24.9 | – | 19.5 | 10 |
| Activating | 100 | 5.5 | 6.8 | – | – | 25 | 19.5 | 10 |
TES, N-tris[hydroxymethyl]methyl-2-aminoethanesulphonic acid; ATP, adenosine 5′-triphosphate; EGTA, ethylene glycol-bis-(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid; HDTA, 1,6 diaminohexane-N,N,N′,N′-tetraacetic acid; CP, N-[Imino(phosphonoamino)methyl]-N-methylglycine; GLH, glutathione. 1 mg ml−1 creatine phosphokinase, 10-μm trans-epoxysuccinyl-l-leucylamido-(4-guanidino)butane (E-64) and 20 μg ml−1 leupeptin were added to all solutions. pH of all solutions, 7.10 at 12°C (adjusted with KOH, 2–12 × 10−3m). Ionic strength: 200 mm. HDTA was obtained from Fluka (Buchs, Switzerland). All other chemicals were obtained from Sigma.