Figure 5. Effects of H-89 (a PKA inhibitor) and forskolin.

CBF ratios (•) are plotted on the left y-axis and V/V₀ values (⋄ (red)) are on the right y-axis. A, H-89 did not induce any changes in CBF, although it decreased V/V₀ slightly in unstimulated cells. The subsequent stimulation with terbutaline did not induce any changes in CBF or V/V₀. B, 10 μm forskolin (FK) decreased CBF and V/V₀ to an extent comparable with 10 μm terbutaline. *Paired values significantly different from those at time 0 (P < 0.05).