FIG. 5.

Gel mobility shift analysis of NanR. (A) Extract of JM101 harboring pTZ(Nan1-3) was added to the 246-bp labeled DNA fragment and subjected to gel shift analysis. Lane 1, DNA fragment alone; lanes 2 to 7, serial 10-fold dilutions of the extract (0.5 mg of total protein/ml) starting at a 1/10 dilution. (B) Gel shift analysis with extract of the nanR7 mutant IBPC1017 (lane 2) and with the exogenous addition of 26 nM NanR-His₆ (lane 3). Lane 1, DNA fragment alone. (C) Gel shift activity using purified NanR-His₆ was determined at different dilutions. Lane 1, DNA fragment alone. Lanes 2 to 10 contained 44.2, 17.7, 8.8, 6.6, 4.5, 2.2 (duplicate lanes), 1.7, and 0.8 nM NanR-His₆, respectively.