TABLE 1.
Carbon sourcea | Relevant genotype | β-Galactosidase activity (Miller units) | Fold induction |
---|---|---|---|
None | nanR+ | 25 ± 9 | 1 |
Glucose | nanR+ | 5 ± 3 | 0.2 |
GlcNAc | nanR+ | 20 ± 2 | 0.7 |
GlcN | nanR+ | 50 ± 20 | 2 |
ManNAc | nanR+ | 50 ± 15 | 2 |
Man | nanR+ | 100 ± 30 | 4 |
Neu5Ac | nanR+ | 5,300 ± 950 | 212 |
Glucoseb | nanR6 | 3,300 ± 350 | 132 |
Glycerolb | nanR6 | 7,850 ± 450 | 314 |
Glucoseb | nanR7 | 2,500 ± 160 | 100 |
Glycerolb | nanR7 | 8,300 ± 990 | 332 |
Unless otherwise indicated, strain JM101 bearing a nanA-lacZ fusion was grown in minimal E medium and 0.5% Casamino Acids with glycerol as the carbon source at 0.4% final concentration or with the indicated sugar added to a final concentration of 2 mg/ml. The results are the averages of three independent measurements±standard deviations.
JM101 carrying the nanA-lacZ fusion and either nanR6 or nanR7 was grown in MOPS (morpholine propanesulfonic acid) medium at 30°C with 0.2% glucose or 0.4% glycerol. The results are the means of two independent cultures. Abbreviations are defined in the text.