TABLE 3.
Binding of NanR to artificial operators with various numbers of tandem GGTATA repeats
| Operatora | Length (bp) | No. of tandem repeats | No. of bands in gel shift |
|---|---|---|---|
| 5′-TAATTGATCTGGTATATCGTTTATCAGA-3′ | 28 | 1 | 1 or 2 |
| 5′-TAATTGATCTGGTATAACAGGTATATCGTTTATCAGA-3′ | 37 | 2 | 2 |
| 5′-CTTAATTGATCTGGTATAACAGGTATAAAGGTATATCGTTTATACGACA-3′ | 49 | 3 | 3 |
| 5′-GGTGTTGCTTAATTGATCTGGTATAACAGGTATAAAGGTATATCGTTTATCAGACAAGCA-3′ | 60 | 3 | 3 |
| 5′-TTGATCTGGTATAACAGGTATAAAGGTATAAAGGTATATCGTTTA-3′ | 45 | 4 | 4 or 5 |
| 5′-TTGATCTGGTATAACAGGTATAAAGGTATAAAGGTATAAAGGTATAAAGGTATATCGTTT-3′ | 60 | 6 | 6 or 7 |
a
Only the 5′ strand of each operator is shown, with the GGTATA motifs underlined.