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. 2003 Dec 12;555(Pt 1):205–218. doi: 10.1113/jphysiol.2003.046474

Figure 2. RT-PCR characterization of NBCn1 message extracted from 10 single mTAL tubule segments.

Figure 2

Two different primer sets were used (Table 2) to investigate which splice variant is expressed in rat mTAL. The longer transcript in A (207 bp) reflects a message which does not contain the information of exon 7 but that of the so-called ‘A-cassette’. The shorter amplicon in A does not contain the A-cassette. The second primer set (B, Table 2) identified the presence of the so-called ‘B-cassette’ in renal NBCn1. In D the full length NBCn1 and the two different renal splice variants are depicted schematically. Also seen in Fig. 3C are data showing the specific message for BTR1 in single isolated mTAL tubules.