Table 1.
Exerimental solutions
| Component | No. 1: Hepes | No. 2: Hepes Na+ free HCO3− free | No. 3: HCO3− Na+ free | No. 4: HCO3− Cl− free | No. 5: HCO3− Cl− free | No. 6: HCO3− Na+ free | No. 7: Hepes Cl− free, Na+ free |
|---|---|---|---|---|---|---|---|
| NaCl | 145 | — | 118 | — | — | — | — |
| NMDG | — | 145 | — | 120 | — | 119 | 145 |
| KH2PO4 | 0.4 | 0.4 | 0.4 | 0.4 | 0.4 | 0.23 | 0.4 |
| K2HPO4 | 1.6 | 1.6 | 1.6 | 1.6 | 1.6 | 0.95 | 1.6 |
| Glucose | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
| Hepes | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
| MgCl2 | 1 | 1 | 1 | 1 | — | — | — |
| MgSO4 | — | — | — | — | — | 6 | — |
| Ca gluconate | 1.3 | 1.3 | 1.3 | 1.3 | 8 | 4 | 4 |
| EDTA | — | — | — | — | — | 0.026 | — |
| NaHCO3− | — | — | 24 | — | 24 | — | — |
| HCl | — | 130 | — | 100 | — | — | — |
| Choline-HCO3− | — | — | — | 24 | — | 24 | — |
| Na gluconate | — | — | — | — | 121 | — | — |
| Gluconic acid | — | — | — | — | — | 121 | 145 |
All solutions had a pH of 7.40 prior to experiments. All solutions were gassed with carbogen (5% CO2–95 %O2) except for solutions 1 and 7.